A blast without power - cell death induced by the tuberculosis-necrotizing toxin fails to elicit adequate immune responses.

ABSTRACT: In this study, we deploy a doxycycline-dependent suicide switch integrated in a tumor challenge model. With this experimental setup, we characterized the immunological consequences of cells dying by four distinct cell death stimuli in vivo. We observed that apoptotic cell death induced by expression of the truncated form of BH3 interacting-domain death agonist (tBid) and a constitutively active form of caspase 3 (revC3), respectively, showed higher immunogenicity than cell death induced by expression of the tuberculosis-necrotizing toxin (TNT). Our data indicate that the early release of ATP induces the silent clearance of dying cells, whereas the simultaneous presence of 'find me' signals and danger-associated molecular patterns (DAMPs) promotes inflammatory reactions and increased immuno

SUBMITTER: Maueroder C 

PROVIDER: S-EPMC4987737 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature