Novel Group of Leaderless Multipeptide Bacteriocins from Gram-Positive Bacteria.

ABSTRACT:

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From raw milk we found 10 Lactococcus garvieae isolates that produce a new broad-spectrum bacteriocin. Though the isolates were obtained from different farms, they turned out to possess identical inhibitory spectra, fermentation profiles of sugars, and repetitive sequence-based PCR (rep-PCR) DNA patterns, indicating that they produce the same bacteriocin. One of the isolates (L. garvieae KS1546) was chosen for further assessment. Purification and peptide sequencing combined with genome sequencing revealed that the antimicrobial activity was due to a bacteriocin unit composed of three similar peptides of 32 to 34 amino acids. The three peptides are produced without leader sequences, and their genes are located next to each other in an operon-like structure, adjacent to th

SUBMITTER: Ovchinnikov KV 

PROVIDER: S-EPMC4988205 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature