LPEseq: Local-Pooled-Error Test for RNA Sequencing Experiments with a Small Number of Replicates.

ABSTRACT: RNA-Sequencing (RNA-Seq) provides valuable information for characterizing the molecular nature of the cells, in particular, identification of differentially expressed transcripts on a genome-wide scale. Unfortunately, cost and limited specimen availability often lead to studies with small sample sizes, and hypothesis testing on differential expression between classes with a small number of samples is generally limited. The problem is especially challenging when only one sample per each class exists. In this case, only a few methods among many that have been developed are applicable for identifying differentially expressed transcripts. Thus, the aim of this study was to develop a method able to accurately test differential expression with a limited number of samples, in particular non-repli

SUBMITTER: Gim J 

PROVIDER: S-EPMC4988759 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature