Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India.

ABSTRACT:

Background & objectives

Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of Brucella. The present study describes the isolation and biochemical, molecular characterization of Brucella melitensis from patients suspected for human brucellosis.

Methods

The blood samples were collected from febrile patients suspected to have brucellosis. A total of 18 isolates were obtained from 102 blood samples subjected to culture. The characterization of these 18 isolates was done by growth on Brucella specific medium, biochemical reactions, CO2 requirement, H2S production, agglutination with A and M mono-specific antiserum, dye sensitivity to basic fuchsin and thionin. Further,

SUBMITTER: Barua A 

PROVIDER: S-EPMC4989840 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature