Wide-field quantitative imaging of tissue microstructure using sub-diffuse spatial frequency domain imaging.

ABSTRACT: Localized measurements of scattering in biological tissue provide sensitivity to microstructural morphology but have limited utility to wide-field applications, such as surgical guidance. This study introduces sub-diffusive spatial frequency domain imaging (sd-SFDI), which uses high spatial frequency illumination to achieve wide-field sampling of localized reflectances. Model-based inversion recovers macroscopic variations in the reduced scattering coefficient [Formula: see text] and the phase function backscatter parameter (γ). Measurements in optical phantoms show quantitative imaging of user-tuned phase-function-based contrast with accurate decoupling of parameters that define both the density and the size-scale distribution of scatterers. Measurements of fresh ex vivo breast tis

SUBMITTER: McClatchy DM 

PROVIDER: S-EPMC4989924 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature