Characteristics of cardiac allograft vasculopathy induced by immunomodulation in the miniature Swine.

ABSTRACT:

Purpose

We aimed to develop swine cardiac transplantation model for study of cardiac allograft vasculopathy (CAV) and to characterize the mechanisms of its formation.

Methods

Heterotropic cardiac transplantation was performed in swine leukocyte antigen mismatched miniature swine, and CAV was induced by immunomodulation by cyclosporine A (CyA). Histology and immunohistochemistry were performed to identify cellular components of CAV. Fluorescence in situ hybridization (FISH) was developed for detection of 1 and Y-chromosome for identification of cell origin in the female donor to the male recipient heart transplantation model.

Results

CAV was successfully developed by immunomodulation of CyA. Severity of CAV revealed more prominent in the distal epicardial coronary arte

SUBMITTER: Amano J 

PROVIDER: S-EPMC4989986 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature