A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.

ABSTRACT: Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference genes, selected for the lowest variation in representative samples, can be used to control random technical variation. Reference gene assays must be reliable, have high amplification specificity and efficiency, and not produce signals from contaminating DNA. Whilst recent research papers identify specific genes that are stable in particular tissues and experimental treatments, here we describe a panel of ten avian gene primer and probe sets that can be used to identify suitable reference genes in many experimental contexts. The panel was tested with TaqMan and SYBR Green systems in two experimental scenarios: a tissue collection and virus i

SUBMITTER: Staines K 

PROVIDER: S-EPMC4990416 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature