A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines.

ABSTRACT: More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.

SUBMITTER: Ogura Y 

PROVIDER: S-EPMC4990470 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature