A quantitative metabolomics profiling approach for the noninvasive assessment of liver histology in patients with chronic hepatitis C.

ABSTRACT:

Background

High-throughput technologies have the potential to identify non-invasive biomarkers of liver pathology and improve our understanding of basic mechanisms of liver injury and repair. A metabolite profiling approach was employed to determine associations between alterations in serum metabolites and liver histology in patients with chronic hepatitis C virus (HCV) infection.

Methods

Sera from 45 non-diabetic patients with chronic HCV were quantitatively analyzed using (1)H-NMR spectroscopy. A metabolite profile of advanced fibrosis (METAVIR F3-4) was established using orthogonal partial least squares discriminant analysis modeling and validated using seven-fold cross-validation and permutation testing. Bioprofiles of moderate to severe steatosis (≥33 %) and necroinflam

SUBMITTER: Sarfaraz MO 

PROVIDER: S-EPMC4990529 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature