In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains.

ABSTRACT:

Background

In animals with bilateral symmetry, midline crossing of axons in the developing central nervous system is regulated by Slit ligands and their neuronal Roundabout (Robo) receptors. Multiple structural domains are present in an evolutionarily conserved arrangement in Robo family proteins, but our understanding of the functional importance of individual domains for midline repulsive signaling is limited.

Methods

We have examined the functional importance of each of the five conserved immunoglobulin-like (Ig) domains within the Drosophila Robo1 receptor. We generated a series of Robo1 variants, each lacking one of the five Ig domains (Ig1-5), and tested each for their ability to bind Slit when expressed in cultured Drosophila cells. We used a transgenic approach to ex

SUBMITTER: Reichert MC 

PROVIDER: S-EPMC4991095 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature