A Macrophage Infection Model to Predict Drug Efficacy Against Mycobacterium Tuberculosis.

ABSTRACT: In the last 40 years, only a single new antituberculosis drug was FDA approved. New tools that improve the drug development process will be essential to accelerate the development of next-generation antituberculosis drugs. The drug development process seems to be hampered by the inefficient transition of initially promising hits to candidate compounds that are effective in vivo. In this study, we introduce an inexpensive, rapid, and BSL-2 compatible infection model using macrophage-passaged Mycobacterium tuberculosis (Mtb) that forms densely packed Mtb/macrophage aggregate structures suitable for drug efficacy testing. Susceptibility to antituberculosis drugs determined with this Mtb/macrophage aggregate model differed from commonly used in vitro broth-grown single-cell Mtb cultures. Impor

SUBMITTER: Schaaf K 

PROVIDER: S-EPMC4991579 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature