Investigating the Interplay between Sister Chromatid Cohesion and Homolog Pairing in Drosophila Nuclei.

ABSTRACT: Following DNA replication, sister chromatids must stay connected for the remainder of the cell cycle in order to ensure accurate segregation in the subsequent cell division. This important function involves an evolutionarily conserved protein complex known as cohesin; any loss of cohesin causes premature sister chromatid separation in mitosis. Here, we examined the role of cohesin in sister chromatid cohesion prior to mitosis, using fluorescence in situ hybridization (FISH) to assay the alignment of sister chromatids in interphase Drosophila cells. Surprisingly, we found that sister chromatid cohesion can be maintained in G2 with little to no cohesin. This capacity to maintain cohesion is widespread in Drosophila, unlike in other systems where a reduced dependence on cohesin for sister chr

SUBMITTER: Senaratne TN 

PROVIDER: S-EPMC4991795 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature