Screening Reliable Reference Genes for RT-qPCR Analysis of Gene Expression in Moringa oleifera.

ABSTRACT: Moringa oleifera is a promising plant species for oil and forage, but its genetic improvement is limited. Our current breeding program in this species focuses on exploiting the functional genes associated with important agronomical traits. Here, we screened reliable reference genes for accurately quantifying the expression of target genes using the technique of real-time quantitative polymerase chain reaction (RT-qPCR) in M. oleifera. Eighteen candidate reference genes were selected from a transcriptome database, and their expression stabilities were examined in 90 samples collected from the pods in different developmental stages, various tissues, and the roots and leaves under different conditions (low or high temperature, sodium chloride (NaCl)- or polyethyleneglycol (PEG)- simulated wat

SUBMITTER: Deng LT 

PROVIDER: S-EPMC4991797 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature