Optimizing preservation of extracellular vesicular miRNAs derived from clinical cerebrospinal fluid.

ABSTRACT:

Background

Tumor specific genetic material can be detected in extracellular vesicles (EVs) isolated from blood, cerebrospinal fluid (CSF), and other biofluids of glioblastoma patients. As such, EVs have emerged as a promising platform for biomarker discovery. However, the optimal procedure to transport clinical EV samples remains poorly characterized.

Methods

We examined the stability of EVs isolated from CSF of glioblastoma patients that were stored under different conditions. EV recovery was determined by Nanoparticle tracking analysis, and qRT-PCR was performed to determine the levels of miRNAs.

Results

CSF EVs that were lyophilized and stored at room temperature (RT) for seven days exhibited a 37-43% reduction in EV number. This reduction was further associated wi

SUBMITTER: Akers JC 

PROVIDER: S-EPMC4992418 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature