Identification of cellular targets of a series of boron heterocycles using TIPA II-A sensitive target identification platform.

ABSTRACT: One of the hurdles in the discovery of antibiotics is the difficulty of linking antibacterial compounds to their cellular targets. Our laboratory has employed a genome-wide approach of over-expressing essential genes in order to identify cellular targets of antibacterial inhibitors. Our objective in this project was to develop and validate a more sensitive disk diffusion based platform of target identification (Target Identification Platform for Antibacterials version 2; TIPA II) using a collection of cell clones in an Escherichia coli mutant (AS19) host with increased outer membrane permeability. Five known antibiotics/inhibitors and 28 boron heterocycles were tested by TIPA II assay, in conjunction with the original assay TIPA. The TIPA II was more sensitive than TIPA because eight boron

SUBMITTER: Ward MS 

PROVIDER: S-EPMC4992540 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature