Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing.

ABSTRACT: Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number aberrations (CNAs) smaller than 3 Mb. A recent WGA method, called TruePrime single cell WGA, uses a recently discovered DNA primase, TthPrimPol, instead of artificial primers to initiate DNA amplification. This method could lead to a lower representation bias, and consequently to a better detection of CNAs. The enzyme requires no complementarity and thus should generate random primers, equally distributed across the genome. The performance of TruePrime WGA was assessed for aneuploidy screening and CNA analysis after MPS, starting from 1, 3

SUBMITTER: Deleye L 

PROVIDER: S-EPMC4992833 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature