Increasing the performance of pooled CRISPR-Cas9 drop-out screening.

ABSTRACT: Components of the type II CRISPR-Cas complex in bacteria have been used successfully in eukaryotic cells to facilitate rapid and accurate cell line engineering, animal model generation and functional genomic screens. Such developments are providing new opportunities for drug target identification and validation, particularly with the application of pooled genetic screening. As CRISPR-Cas is a relatively new genetic screening tool, it is important to assess its functionality in a number of different cell lines and to analyse potential improvements that might increase the sensitivity of a given screen. To examine critical aspects of screening quality, we constructed ultra-complex libraries containing sgRNA sequences targeting a collection of essential genes. We examined the performance of sc

SUBMITTER: Cross BC 

PROVIDER: S-EPMC4992892 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature