Salmonella Rapidly Regulates Membrane Permeability To Survive Oxidative Stress.

ABSTRACT:

Unlabelled

The outer membrane (OM) of Gram-negative bacteria provides protection against toxic molecules, including reactive oxygen species (ROS). Decreased OM permeability can promote bacterial survival under harsh circumstances and protects against antibiotics. To better understand the regulation of OM permeability, we studied the real-time influx of hydrogen peroxide in Salmonella bacteria and discovered two novel mechanisms by which they rapidly control OM permeability. We found that pores in two major OM proteins, OmpA and OmpC, could be rapidly opened or closed when oxidative stress is encountered and that the underlying mechanisms rely on the formation of disulfide bonds in the periplasmic domain of OmpA and TrxA, respectively. Additionally, we found that a Salmonella mutant

SUBMITTER: van der Heijden J 

PROVIDER: S-EPMC4992977 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature