Unbiased analysis of senescence associated secretory phenotype (SASP) to identify common components following different genotoxic stresses.

ABSTRACT: Senescent cells secrete senescence-associated secretory phenotype (SASP) proteins to carry out several functions, such as sensitizing surrounding cells to senesce; immunomodulation; impairing or fostering cancer growth; and promoting tissue development. Identifying secreted factors that achieve such tasks is a challenging issue since the profile of secreted proteins depends on genotoxic stress and cell type. Currently, researchers are trying to identify common markers for SASP. The present investigation compared the secretome composition of five different senescent phenotypes in two different cell types: bone marrow and adipose mesenchymal stromal cells (MSC). We induced MSC senescence by oxidative stress, doxorubicin treatment, X-ray irradiation, and replicative exhaustion. We took advant

SUBMITTER: Ozcan S 

PROVIDER: S-EPMC4993333 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature