From Constructs to Crystals - Towards Structure Determination of β-barrel Outer Membrane Proteins.

ABSTRACT: Membrane proteins serve important functions in cells such as nutrient transport, motility, signaling, survival and virulence, yet constitute only ~1% percent of known structures. There are two types of membrane proteins, α-helical and β-barrel. While α-helical membrane proteins can be found in nearly all cellular membranes, β-barrel membrane proteins can only be found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria. One common bottleneck in structural studies of membrane proteins in general is getting enough pure sample for analysis. In hopes of assisting those interested in solving the structure of their favorite β-barrel outer membrane protein (OMP), general protocols are presented for the production of target β-barrel OMPs at levels useful for structure

SUBMITTER: Noinaj N 

PROVIDER: S-EPMC4993350 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature