Environmental Transmission of the Gut Symbiont Burkholderia to Phloem-Feeding Blissus insularis.

ABSTRACT: The plant-phloem-feeding Blissus insularis possesses specialized midgut crypts, which harbor a dense population of the exocellular bacterial symbiont Burkholderia. Most individual B. insularis harbor a single Burkholderia ribotype in their midgut crypts; however, a diverse Burkholderia community exists within a host population. To understand the mechanism underlying the consistent occurrence of various Burkholderia in B. insularis and their specific association, we investigated potential gut symbiont transmission routes. PCR amplification detected a low titer of Burkholderia in adult reproductive tracts; however, fluorescence in situ hybridization assays failed to produce detectable signals in these tracts. Furthermore, no Burkholderia-specific PCR signals were detected in eggs and neonate

SUBMITTER: Xu Y 

PROVIDER: S-EPMC4993365 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature