Single-Molecule FISH Reveals Non-selective Packaging of Rift Valley Fever Virus Genome Segments.

ABSTRACT: The bunyavirus genome comprises a small (S), medium (M), and large (L) RNA segment of negative polarity. Although genome segmentation confers evolutionary advantages by enabling genome reassortment events with related viruses, genome segmentation also complicates genome replication and packaging. Accumulating evidence suggests that genomes of viruses with eight or more genome segments are incorporated into virions by highly selective processes. Remarkably, little is known about the genome packaging process of the tri-segmented bunyaviruses. Here, we evaluated, by single-molecule RNA fluorescence in situ hybridization (FISH), the intracellular spatio-temporal distribution and replication kinetics of the Rift Valley fever virus (RVFV) genome and determined the segment composition of mature v

SUBMITTER: Wichgers Schreur PJ 

PROVIDER: S-EPMC4993503 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature