RNA-seq analysis of clinical-grade osteochondral allografts reveals activation of early response genes.

ABSTRACT: Preservation of osteochondral allografts used for transplantation is critical to ensure favorable outcomes for patients after surgical treatment of cartilage defects. To study the biological effects of protocols currently used for cartilage storage, we investigated differences in gene expression between stored allograft cartilage and fresh cartilage from living donors using high throughput molecular screening strategies. We applied next generation RNA sequencing (RNA-seq) and real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) to assess genome-wide differences in mRNA expression between stored allograft cartilage and fresh cartilage tissue from living donors. Gene ontology analysis was used to characterize biological pathways associated with differentially expr

SUBMITTER: Lin Y 

PROVIDER: S-EPMC4993686 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature