Hydrogen sulfide metabolism regulates endothelial solute barrier function.

ABSTRACT: Hydrogen sulfide (H₂S) is an important gaseous signaling molecule in the cardiovascular system. In addition to free H₂S, H₂S can be oxidized to polysulfide which can be biologically active. Since the impact of H₂S on endothelial solute barrier function is not known, we sought to determine whether H₂S and its various metabolites affect endothelial permeability. In vitro permeability was evaluated using albumin flux and transendothelial electrical resistance. Different H₂S donors were used to examine the effects of exogenous H₂S. To evaluate the role of endogenous H₂S, mouse aortic endothelial cells (MAECs) were isolated from wild type mice and mice lacking cystathionine γ-lyase (CSE), a predominant sourc

SUBMITTER: Yuan S 

PROVIDER: S-EPMC4993857 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature