A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves.

ABSTRACT: We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. This protocol introduces: (1) an efficient method for enzymatic cell release immediately after removal of the epineurium and extensive teasing of the nerve fibers; (2) an adaptable drop-plating method for selective cell attachment, removal of myelin debris, and expansion of the initial SC population in chemically defined medium; (3) a magnetic-activated cell sorting purification protocol for rapid and effective fibroblast elimination; and (4) an optional step of cryopreservation for the storage of

SUBMITTER: Andersen ND 

PROVIDER: S-EPMC4994039 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature