Ontology highlight
ABSTRACT:
SUBMITTER: Maes M
PROVIDER: S-EPMC4994868 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Methods in molecular biology (Clifton, N.J.) 20160101
Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin RNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantif ...[more]