Modeling vitamin D insufficiency and moderate deficiency in adult mice via dietary cholecalciferol restriction.

ABSTRACT:

Purpose

We sought to develop and characterize a model of human vitamin D nutritional insufficiency/deficiency in the adult mouse, which could have broad utility in examining health consequences of this common condition.

Methods

Adult mice were fed diets containing cholecalciferol contents of 0.05 IU/g, 0.25 IU/g, 0.5 IU/g or 1.5 IU/g for four months. We studied induction of steady-state vitamin D insufficiency, and its consequences on primary cholecalciferol metabolite levels, calcium homeostasis, parathyroid physiology, and bone morphology.

Results

All diets were well tolerated, without adverse effects on body weight. Diets containing 0.05 IU/g and 0.25 IU/g cholecalciferol significantly lowered serum 25-hydroxyvitamin D levels (median 25OHD, 10.5 ng/ml, and 21.6 ng/

SUBMITTER: Mallya SM 

PROVIDER: S-EPMC4995161 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature