Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.

ABSTRACT: Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. A number of cytoskeletal proteins are known to be tyrosine phosphorylated (pY) in different cellular processes. However, the profile of pY proteins during different stages of cell spreading has not been available. Using immunoafffinity enrichment of pY proteins coupled with label free quantitative proteomics, we quantitatively identified 447 pY proteins in the migratory ECV-304 cells at the early spreading (adhesion) and the active spreading stages. We found that pY levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading

SUBMITTER: Xie Y 

PROVIDER: S-EPMC4995472 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature