Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis.

ABSTRACT:

Background

Canine atopic dermatitis (cAD) is a common chronic and pruritic skin disease in dogs. The development of cAD involves complex interactions between environmental antigens, genetic predisposition and a number of disparate cell types. The aim of the present study was to perform comprehensive analyses of peripheral blood of AD dogs in relation to healthy subjects in order to determine the changes which would be characteristic for cAD.

Results

The number of cells in specific subpopulations of lymphocytes was analyzed by flow cytometry, concentration of chosen pro- and anti-inflammatory cytokines (IL-4, IL-10, IL-13, TNF-α, TGF-β1) was determined by ELISA; and microarray analysis was performed on RNA samples isolated from peripheral blood nuclear cells of AD and healthy

SUBMITTER: Majewska A 

PROVIDER: S-EPMC4995625 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature