Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli.

ABSTRACT: The phosphotransferase system (PTS) plays a pivotal role in the uptake of multiple sugars in Escherichia coli and many other bacteria. In the cell, individual sugar-specific PTS branches are interconnected through a series of phosphotransfer reactions, thus creating a global network that not only phosphorylates incoming sugars but also regulates a number of cellular processes. Despite the apparent importance of the PTS network in bacterial physiology, the holistic function of the network in the cell remains unclear. Here we used Förster resonance energy transfer (FRET) to investigate the PTS network in E. coli, including the dynamics of protein interactions and the processing of different stimuli and their transmission to the chemotaxis pathway. Our results demonstrate that despite the see

SUBMITTER: Somavanshi R 

PROVIDER: S-EPMC4996493 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature