Greazy: Open-Source Software for Automated Phospholipid Tandem Mass Spectrometry Identification.

ABSTRACT: Lipid identification from data produced with high-throughput technologies is essential to the elucidation of the roles played by lipids in cellular function and disease. Software tools for identifying lipids from tandem mass (MS/MS) spectra have been developed, but they are often costly or lack the sophistication of their proteomics counterparts. We have developed Greazy, an open source tool for the automated identification of phospholipids from MS/MS spectra, that utilizes methods similar to those developed for proteomics. From user-supplied parameters, Greazy builds a phospholipid search space and associated theoretical MS/MS spectra. Experimental spectra are scored against search space lipids with similar precursor masses using a peak score based on the hypergeometric distribution and a

SUBMITTER: Kochen MA 

PROVIDER: S-EPMC4996967 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature