Selection of Reference Genes for Expression Studies of Xenobiotic Adaptation in Tetranychus urticae.

ABSTRACT: Quantitative real-time PCR (qRT-PCR) is an extensively used, high-throughput method to analyze transcriptional expression of genes of interest. An appropriate normalization strategy with reliable reference genes is required for calculating gene expression across diverse experimental conditions. In this study, we aim to identify the most stable reference genes for expression studies of xenobiotic adaptation in Tetranychus urticae, an extremely polyphagous herbivore causing significant yield reduction of agriculture. We chose eight commonly used housekeeping genes as candidates. The qRT-PCR expression data for these genes were evaluated from seven populations: a susceptible and three acaricide resistant populations feeding on lima beans, and three other susceptible populations which had been

SUBMITTER: Morales MA 

PROVIDER: S-EPMC4997057 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature