The emergence of superstructural order in insulin amyloid fibrils upon multiple rounds of self-seeding.

ABSTRACT: Typically, elongation of an amyloid fibril entails passing conformational details of the mother seed to daughter generations of fibrils with high fidelity. There are, however, several factors that can potentially prevent such transgenerational structural imprinting from perpetuating, for example heterogeneity of mother seeds or so-called conformational switching. Here, we examine phenotypic persistence of bovine insulin amyloid ([BI]) upon multiple rounds of self-seeding under quiescent conditions. According to infrared spectroscopy, with the following passages of homologous seeding, daughter fibrils gradually depart from the mother seed's spectral characteristics. We note that this transgenerational structural drift in [BI] amyloid leads toward fibrils with infrared, chiroptical, and morp

SUBMITTER: Surmacz-Chwedoruk W 

PROVIDER: S-EPMC4997315 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature