RNA sequencing from neural ensembles activated during fear conditioning in the mouse temporal association cortex.

ABSTRACT: The stable formation of remote fear memories is thought to require neuronal gene induction in cortical ensembles that are activated during learning. However, the set of genes expressed specifically in these activated ensembles is not known; knowledge of such transcriptional profiles may offer insights into the molecular program underlying stable memory formation. Here we use RNA-Seq to identify genes whose expression is enriched in activated cortical ensembles labeled during associative fear learning. We first establish that mouse temporal association cortex (TeA) is required for remote recall of auditory fear memories. We then perform RNA-Seq in TeA neurons that are labeled by the activity reporter Arc-dVenus during learning. We identify 944 genes with enriched expression in Arc-dVenus+ n

SUBMITTER: Cho JH 

PROVIDER: S-EPMC4997356 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature