Identification of therapeutic targets applicable to clinical strategies in ovarian cancer.

ABSTRACT:

Background

shRNA-mediated lethality screening is a useful tool to identify essential targets in cancer biology. Ovarian cancer (OC) is extremely heterogeneous and most cases are advanced stages at diagnosis. OC has a high response rate initially, but becomes resistant to standard chemotherapy. We previously employed high throughput global shRNA sensitization screens to identify NF-kB related pathways. Here, we re-analyzed our previous shRNA screens in an unbiased manner to identify clinically applicable molecular targets.

Methods

We proceeded with siRNA lethality screening using the top 55 genes in an expanded set of 6 OC cell lines. We investigated clinical relevance of candidate targets in The Cancer Genome Atlas OC dataset. To move these findings towards the clinic, we ch

SUBMITTER: Kim MK 

PROVIDER: S-EPMC4997769 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature