PCR and Genotyping for HPV in Cervical Cancer Patients.

ABSTRACT:

Aims

To devise nested multiplex polymerase chain reaction (NMPCR) protocol for detection of mucosal human papilloma viruses (HPVs) and typing of HPV-16 and -18 in formalin-fixed, paraffin-embedded (FFPE) tissues of carcinoma cervix (CaCx).

Settings and design

Cross-sectional observational study.

Materials and methods

NMPCR was done for simultaneous detection of HPV, targeting 134 bp L1 capsid gene employing GP+/mGP+ primers and typing of genotypes-16 and -18, targeting E6/E7 gene from 34 FFPE tissue blocks of CaCx and cervical intraepithelial neoplasia (CIN). Detection of 142 bp consensus sequence of L1 capsid gene was performed by nested PCR employing MY/GP+ primers. Sequencing of selected PCR amplicons of the later protocol obtained from control cell line DNA and 5

SUBMITTER: Prakash P 

PROVIDER: S-EPMC4997793 | biostudies-literature | 2016 Jul-Sep

REPOSITORIES: biostudies-literature