Whole-Genome Sequencing for Detecting Antimicrobial Resistance in Nontyphoidal Salmonella.

ABSTRACT: Laboratory-based in vitro antimicrobial susceptibility testing is the foundation for guiding anti-infective therapy and monitoring antimicrobial resistance trends. We used whole-genome sequencing (WGS) technology to identify known antimicrobial resistance determinants among strains of nontyphoidal Salmonella and correlated these with susceptibility phenotypes to evaluate the utility of WGS for antimicrobial resistance surveillance. Six hundred forty Salmonella of 43 different serotypes were selected from among retail meat and human clinical isolates that were tested for susceptibility to 14 antimicrobials using broth microdilution. The MIC for each drug was used to categorize isolates as susceptible or resistant based on Clinical and Laboratory Standards Institute clinical breakpoints or N

SUBMITTER: McDermott PF 

PROVIDER: S-EPMC4997858 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature