A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum.

ABSTRACT: The mechanisms underlying the drug resistance of Leishmania spp. are manifold and not completely identified. Apart from the highly conserved multidrug resistance gene family known from higher eukaryotes, Leishmania spp. also possess genus-specific resistance marker genes. One of them, ARM58, was first identified in Leishmania braziliensis using a functional cloning approach, and its domain structure was characterized in L. infantum Here we report that L. infantum ARM58 is part of a gene cluster at the telomeric end of chromosome 34 also comprising the neighboring genes ARM56 and HSP23. We show that overexpression of all three genes can confer antimony resistance to intracellular amastigotes. Upon overexpression in L. donovani, ARM58 and ARM56 are secreted via exosomes, suggesting a scaveng

SUBMITTER: Tejera Nevado P 

PROVIDER: S-EPMC4997884 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature