Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

ABSTRACT: Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

SUBMITTER: Anderson RM 

PROVIDER: S-EPMC4998187 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature