A new patient-derived orthotopic malignant meningioma model treated with oncolytic herpes simplex virus.

ABSTRACT:

Background

Higher-grade meningiomas (HGMs; World Health Organization grades II and III) pose a clinical problem due to high recurrence rates and the absence of effective therapy. Preclinical development of novel therapeutics requires a disease model that recapitulates the genotype and phenotype of patient HGM. Oncolytic herpes simplex virus (oHSV) has shown efficacy and safety in cancers in preclinical and clinical studies, but its utility for HGM has not been well characterized.

Methods

Tumorsphere cultures and serial orthotopic xenografting in immunodeficient mice were used to establish a patient-derived HGM model. The model was pathologically and molecularly characterized by immunohistochemistry, western blot, and genomic DNA sequencing and compared with the patient tumor

SUBMITTER: Nigim F 

PROVIDER: S-EPMC4998993 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature