Tumor Touch Imprints as Source for Whole Genome Analysis of Neuroblastoma Tumors.

ABSTRACT:

Introduction

Tumor touch imprints (TTIs) are routinely used for the molecular diagnosis of neuroblastomas by interphase fluorescence in-situ hybridization (I-FISH). However, in order to facilitate a comprehensive, up-to-date molecular diagnosis of neuroblastomas and to identify new markers to refine risk and therapy stratification methods, whole genome approaches are needed. We examined the applicability of an ultra-high density SNP array platform that identifies copy number changes of varying sizes down to a few exons for the detection of genomic changes in tumor DNA extracted from TTIs.

Material and methods

DNAs were extracted from TTIs of 46 neuroblastoma and 4 other pediatric tumors. The DNAs were analyzed on the Cytoscan HD SNP array platform to evaluate numerical and s

SUBMITTER: Brunner C 

PROVIDER: S-EPMC4999140 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature