The Nucleoid Binding Protein H-NS Biases Genome-Wide Transposon Insertion Landscapes.

ABSTRACT:

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Transposon insertion sequencing (TIS; also known as TnSeq) is a potent approach commonly used to comprehensively define the genetic loci that contribute to bacterial fitness in diverse environments. A key presumption underlying analyses of TIS datasets is that loci with a low frequency of transposon insertions contribute to fitness. However, it is not known whether factors such as nucleoid binding proteins can alter the frequency of transposon insertion and thus whether TIS output may systematically reflect factors that are independent of the role of the loci in fitness. Here, we investigated whether the histone-like nucleoid structuring (H-NS) protein, which preferentially associates with AT-rich sequences, modulates the frequency of Mariner transposon insertion in the

SUBMITTER: Kimura S 

PROVIDER: S-EPMC4999555 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature