The Balance between Recombination Enzymes and Accessory Replicative Helicases in Facilitating Genome Duplication.

ABSTRACT: Accessory replicative helicases aid the primary replicative helicase in duplicating protein-bound DNA, especially transcribed DNA. Recombination enzymes also aid genome duplication by facilitating the repair of DNA lesions via strand exchange and also processing of blocked fork DNA to generate structures onto which the replisome can be reloaded. There is significant interplay between accessory helicases and recombination enzymes in both bacteria and lower eukaryotes but how these replication repair systems interact to ensure efficient genome duplication remains unclear. Here, we demonstrate that the DNA content defects of Escherichia coli cells lacking the strand exchange protein RecA are driven primarily by conflicts between replication and transcription, as is the case in cells lacking t

SUBMITTER: Syeda AH 

PROVIDER: S-EPMC4999830 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature