Experimental Analysis of Functional Variation within Protein Families: Receiver Domain Autodephosphorylation Kinetics.

ABSTRACT:

Unlabelled

Plants and microorganisms use two-component signal transduction systems (TCSs) to mediate responses to environmental stimuli. TCSs mediate responses through phosphotransfer from a conserved histidine on a sensor kinase to a conserved aspartate on the receiver domain of a response regulator. Typically, signal termination occurs through dephosphorylation of the receiver domain, which can catalyze its own dephosphorylation. Despite strong structural conservation between receiver domains, reported autodephosphorylation rate constants (kdephos) span a millionfold range. Variable receiver domain active-site residues D + 2 and T + 2 (two amino acids C terminal to conserved phosphorylation site and Thr/Ser, respectively) influence kdephos values, but the extent and mechanism of

SUBMITTER: Page SC 

PROVIDER: S-EPMC4999930 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature