Reconstitution and Minimization of a Micrococcin Biosynthetic Pathway in Bacillus subtilis.

ABSTRACT:

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Thiopeptides represent one of several families of highly modified peptide antibiotics that hold great promise for natural product engineering. These macrocyclic peptides are produced by a combination of ribosomal synthesis and extensive posttranslational modification by dedicated processing enzymes. We previously identified a compact, plasmid-borne gene cluster for the biosynthesis of micrococcin P1 (MP1), an archetypal thiopeptide antibiotic. In an effort to genetically dissect this pathway, we have reconstituted it in Bacillus subtilis Successful MP1 production required promoter engineering and the reassembly of essential biosynthetic genes in a modular plasmid. The resulting system allows for rapid pathway manipulation, including protein tagging and gene deletion. We

SUBMITTER: Bennallack PR 

PROVIDER: S-EPMC4999933 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature