Functional Human α7 Nicotinic Acetylcholine Receptor (nAChR) Generated from Escherichia coli.

ABSTRACT: Human Cys-loop receptors are important therapeutic targets. High-resolution structures are essential for rational drug design, but only a few are available due to difficulties in obtaining sufficient quantities of protein suitable for structural studies. Although expression of proteins in E. coli offers advantages of high yield, low cost, and fast turnover, this approach has not been thoroughly explored for full-length human Cys-loop receptors because of the conventional wisdom that E. coli lacks the specific chaperones and post-translational modifications potentially required for expression of human Cys-loop receptors. Here we report the successful production of full-length wild type human α7nAChR from E. coli Chemically induced chaperones promote high expression levels of well-folded pro

SUBMITTER: Tillman TS 

PROVIDER: S-EPMC5000075 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature