Cancer Stem Cells in Glioblastoma Multiforme.

ABSTRACT:

Aim

To identify and characterize cancer stem cells (CSC) in glioblastoma multiforme (GBM).

Methods

Four-micrometer thick formalin-fixed paraffin-embedded GBM samples from six patients underwent 3,3-diaminobenzidine (DAB) and immunofluorescent (IF) immunohistochemical (IHC) staining for the embryonic stem cell (ESC) markers NANOG, OCT4, SALL4, SOX2, and pSTAT3. IF IHC staining was performed to demonstrate co-expression of these markers with GFAP. The protein expression and the transcriptional activities of the genes encoding NANOG, OCT4, SOX2, SALL4, and STAT3 were investigated using Western blotting (WB) and NanoString gene expression analysis, respectively.

Results

DAB and IF IHC staining demonstrated the presence of a CSC population expressing NANOG, OCT4, SOX2, SAL

SUBMITTER: Bradshaw A 

PROVIDER: S-EPMC5001191 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature