Transcript degradation and noise of small RNA-controlled genes in a switch activated network in Escherichia coli.

ABSTRACT: Post-transcriptional regulatory processes may change transcript levels and affect cell-to-cell variability or noise. We study small-RNA downregulation to elucidate its effects on noise in the iron homeostasis network of Escherichia coli In this network, the small-RNA RyhB undergoes stoichiometric degradation with the transcripts of target genes in response to iron stress. Using single-molecule fluorescence in situ hybridization, we measured transcript numbers of the RyhB-regulated genes sodB and fumA in individual cells as a function of iron deprivation. We observed a monotonic increase of noise with iron stress but no evidence of theoretically predicted, enhanced stoichiometric fluctuations in transcript numbers, nor of bistable behavior in transcript distributions. Direct detection of Ry

SUBMITTER: Arbel-Goren R 

PROVIDER: S-EPMC5001584 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature