Blue light-mediated transcriptional activation and repression of gene expression in bacteria.

ABSTRACT: Light-regulated modules offer unprecedented new ways to control cellular behavior in precise spatial and temporal resolution. The availability of such tools may dramatically accelerate the progression of synthetic biology applications. Nonetheless, current optogenetic toolbox of prokaryotes has potential issues such as lack of rapid and switchable control, less portable, low dynamic expression and limited parts. To address these shortcomings, we have engineered a novel bidirectional promoter system for Escherichia coli that can be induced or repressed rapidly and reversibly using the blue light dependent DNA-binding protein EL222. We demonstrated that by modulating the dosage of light pulses or intensity we could control the level of gene expression precisely. We show that both light-induc

SUBMITTER: Jayaraman P 

PROVIDER: S-EPMC5001607 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature