Inhibition of G Protein βγ Subunit Signaling Abrogates Nephritis in Lupus-Prone Mice.

ABSTRACT:

Objective

Despite considerable advances in the understanding of systemic lupus erythematosus (SLE), there is still an urgent need for new and more targeted treatment approaches. We previously demonstrated that small-molecule blockade of G protein βγ subunit (Gβγ) signaling inhibits acute inflammation through inhibition of chemokine receptor signal transduction. We undertook this study to determine whether inhibition of Gβγ signaling ameliorates disease in a mouse model of SLE.

Methods

Lupus-prone (NZB × NZW)F1 female mice were prophylactically or therapeutically treated with the small-molecule Gβγ inhibitor gallein. Tissue samples were analyzed by flow cytometry and immunohistochemistry. The development and extent of nephritis were assessed by monitoring proteinuria and by i

SUBMITTER: Rangel-Moreno J 

PROVIDER: S-EPMC5001921 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature